Twinbee Portable compiles five different Twinbee games on the PSP:
- The arcade version of the original Twinbee
- A version of Twinbee da! with redrawn graphics
- The arcade version of Deta na! Twinbee
- Pop 'n Twinbee
- The arcade game Twinbee Yahoo!
In order to reproduce the original arcade games as closely as possible, the PSP can be rotated 90 degrees when playing those games replicated in their coin-op version. Twinbee da, originally made for the Gameboy, has had its graphics redrawn in full colour and to fit the high resolution of the PSP screen. Stage 3 of the original GB version has been left out, though.